Filliaux Industries Data Analysis Services
Request a quote

Quote

Request a quote

Tell us what you are trying to measure and send one to ten representative images. They run through the analysis pipeline for your sample type and are reviewed by a person, so the reply says whether the measurement is feasible on your actual data, what the feasibility assessment would involve, and what it would cost.

Before you send

  • Send the instrument's raw files. Exported PNG or JPEG pictures have lost the height data every measurement depends on.
  • Representative beats best: a typical field tells you more than the cleanest one.
  • Up to 10 images, 250 MB each.
  • Nothing here commits you to anything. If the measurement looks feasible, the next step is a feasibility assessment at $500, credited in full against the project.

What can be measured

Three sample types have a validated pipeline

Pick the matching sample type in the form and your images go through that pipeline, with its own measurement rules and QC galleries. Anything else is looked at individually.

Validated pipeline

Nucleosomes or chromatin on DNA

Wrapped length, free arm lengths, nucleosome height and volume, nucleosome versus chromatosome, positioning on the DNA.

Say in the form: The DNA construct (length in bp, positioning sequence) and the histones or linker histone used.

Validated pipeline

A protein bound to DNA

Fraction of DNA bound, copies per molecule, bound protein volume and height, binding position along the DNA, DNA bend angle.

Say in the form: The construct and binding-site positions, the protein and its expected size. A DNA-only image is the most useful extra file you can send.

Validated pipeline

Amyloid-beta aggregates

Aggregate height and volume, size distribution, morphology and elongation, surface loading, oligomer size in monomer equivalents.

Say in the form: Peptide concentration, substrate (bilayer composition or mica) and incubation time.

Assessed individually

Something else

Particles, vesicles, fibrils, other DNA or protein samples. These are looked at individually before anything runs.

Say in the form: What the sample is and what you need measured, as precisely as you can.

The confirmation and the reply go here, so please check it is correct.

Nucleosome, protein–DNA and amyloid samples go straight into a validated pipeline.

How you refer to it, e.g. "M1 + H3 octamer, 1:1, batch 2".

About the sample

The pipeline for your sample type needs a few facts up front; the questions change with the sample type you chose. Anything you leave out is asked for in the reply, so it only slows things down.

Its name or a one-line description, e.g. "M1, pUC19-derived PCR fragment" or "601 array, 12 × 197 bp".

Base pairs of the construct as imaged. Sets the contour-length ruler the measurements are calibrated against.

E.g. Widom 601, 5S rDNA, MMTV, or "none / random". Where it sits on the construct if you know it.

E.g. "H3/H4/H2A/H2B octamer (recombinant Xenopus)", "+ linker histone H1.0". Variants and tags matter for size.

Name and subunits, e.g. "NF-κB p50/p65 heterodimer, ~90 kDa" or "E. coli SSB tetramer". Mass per copy sets the expected volume.

How many protein copies you expect on one DNA molecule when it is bound. "Unknown" is fine.

Position from one end (e.g. "κB site at 410 bp of 1000"), the sequence, or "non-specific". Positions are reported relative to this.

What the peptide was deposited on. A bilayer changes the background and the aggregate shapes the detection expects.

E.g. "DOPC:DOPS 7:3", "POPC + 20 mol% cholesterol", "total brain lipid extract".

The series you imaged, e.g. "protein:DNA 0, 0.5, 1, 2" or "1 µM Aβ42 at 0, 2, 6 h", and how each folder or filename says which condition it is ("..._r1_..." = ratio 1).

A DNA-only (or peptide-free) prep imaged the same way lets us calibrate the detection and report a false-positive rate. Say yes if one of the images you send is that control.

Concentrations at deposition, buffer and divalent cations, deposition time, rinse and drying (or imaging in liquid). Whatever the card above asks for that is not covered above.

Scan size divided by pixels per line, e.g. 2000 nm / 1024 = 1.95. Only if you know it; the raw files usually carry it.

The side of a typical field, e.g. 2 for a 2 × 2 µm scan.

What do you need measured

Tick everything that applies. Each distinct measurement is a measurand in the quote.

DNA

Protein–DNA

Nucleosomes and chromatin

Aggregates and fibrils

Particles and vesicles

Something else

What you want back (optional)

Pre-ticked for your sample type; change it to suit. Figures are publication-style panels; raw tables are the per-object CSVs behind every number.

It drives the estimate more than anything else on this form.

Representative images

Between 1 and 10 raw instrument files, up to 250 MB each. Typical fields, not just the best ones.

    Deadlines, a grant aim this supports, a reviewer request you are responding to, or constraints on how the data can be handled.

    You will receive an automatic confirmation by email straight away.

    Afterwards

    What happens after you send this

    1. You get an automatic confirmation by email straight away, with a reference number and a copy of what you sent.
    2. A person checks the images and the request before anything runs: format, scan size, and whether the sample fits a pipeline.
    3. The images go through that pipeline: detection, then QC galleries of every object it found and every field it traced, reviewed by hand.
    4. You get a personal reply saying whether the measurement is feasible on your data. If it is, you are quoted a fixed fee for a feasibility assessment.
    5. The assessment tells you how many measurable objects your full dataset contains, and produces a firm fixed price for the full run.

    If an assessment is already agreed and you need to send the full dataset, see how to send data.

    Data handling

    How your images are handled

    Uploaded over HTTPS

    Images go to storage on the server that hosts this site and are moved to the analysis machine within minutes, after which the server's copy is deleted.

    Used only for your request

    Your images are not used for any other purpose without written agreement.

    90-day retention

    Raw inputs are kept for 90 days after the reply and then removed. An NDA can be put in place on request before you upload.